COG6-CDG: Two Novel Variants and Milder Phenotype in a Chinese Patient

Table 1

Primers used for effects of variants on RNA decay or splicing.

Amplified region	Primer name	Sequence (5-3)	Length of PCR product (WT^a/Mut)
COG6_exons 15-18	c1672_F	TCATGTGTCTTGGATCCTCTCC	402 bp	402 bp
	c1672_R	CACTGTGGCACTTAGAAGAAAGT
COG6_exon 1-3	c153_F	TGCCAACGGCCTCAACAAGG	263 bp	417 bp
	c153_R	TGCTTTCAAGTTCCTCCTTCACT	328 bp	482 bp

^aThere are two wild-type (WT) manuscripts (NM_020751.3, 263 bp, and NR_026745.1, 328 bp), owing to an additional splicing site in intron 1.