Chlamydia trachomatis Immune Evasion via Downregulation of MHC Class I Surface Expression Involves Direct and Indirect Mechanisms

Figure 2

Separation of infected and uninfected endocervical epithelial cells in a C. trachomatis-exposed culture. Using chlamydial-LPS fluorescently labeled with FITC, infected and noninfected A2EN cell populations from the same culture can be distinguished and analyzed independently. This separation allows for the analysis of specific host cellular responses to C. trachomatis infection in infected and in bystander-uninfected cells. Left column shows side scatter (SSC) and chlamydial LPS-FITC dot plots, indicating a shift in granularity of FITC positive cells. Right column shows cell number and chlamydial-LPS histograms, which reflect the proportion of uninfected and C. trachomatis-infected cells within a pool of cells from A2En cultures exposed to varying multiplicities of infection (MOIs). Data is representative of experiments performed greater than 10 times.