Chlamydia trachomatis Immune Evasion via Downregulation of MHC Class I Surface Expression Involves Direct and Indirect Mechanisms

Figure 4

Effect of C. trachomatis-exposed A2EN cell culture media on MHC class I surface expression on uninfected A2EN endocervical epithelial cells. Uninfected monolayers of A2EN cells were exposed to culture media from C. trachomatis-infected A2EN cell culture (green), mock-infected culture (gray), IFN gamma exposed infected cell culture (blue), and fresh culture media (red). An isotype control is shown as black line histogram. Exposure of endocervical cells to culture media from C. trachomatis-infected culture at an MOI of 2 for 38 hours, (during which ~50% of cells are infected with C. trachomatis) resulted in the downregulation of MHC class I expression on endocervical epithelial cells. Data is representative of experiments performed twice in duplicate.