Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay: Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases

Table 2

Demographic, clinicopathological findings, and case-by-case comparison of IHC, PCR, and microsphere assay results.

Case no.	Age	Association with pregnancy	Outcome	Tissue tested	Pathological findings	Clostridia IHC	C. sordellii PCR	C. perfringens PCR	Microsphere assay
1	18	Medical abortion	Fatal	Uterus	Necrotizing endomyometritis	Positive	Positive	Negative	Positive for C. sordellii
2	22	Medical abortion	Fatal	Uterus	Necrotizing endomyometritis	Positive	Positive	Negative	Positive for C. sordellii
3	34	Medical abortion	Fatal	Uterus	Necrotizing endomyometritis	Positive	Positive	Negative	Positive for C. sordellii
4	21	Medical abortion	Fatal	Uterus	Necrotizing endomyometritis	Positive	Positive	Negative	Positive for C. sordellii
5	26	Medical abortion	Fatal	Endometrium	Necrotizing endomyometritis	Positive	Positive	Negative	Positive for C. sordellii
6	25	Spontaneous abortion	Non-fatal	Placenta	Severe acute chorioamnionitis	Positive	Positive	Negative	Positive for C. sordellii
7	32	No association	Fatal	Uterus	Necrotizing endometritis	Positive	Positive	Negative	Positive for C. sordellii
8	18	Medical abortion	Fatal	Unknown	Necrotizing endometritis	Positive	Positive	Negative	Positive for C. sordellii
9	29	Medical abortion	Fatal	Uterus	Necrotizing endometritis	Positive	Positive	Negative	Positive for C. sordellii
10	21	Medical abortion	Fatal	Decidual and chorionic tissue	Acute inflammation and necrosis	Positive	Positive	Negative	Positive for C. sordellii
11	24	Medical abortion	Fatal	Uterus	Necrotizing endomyometritis	Positive	Negative	Positive	Positive for C. perfringens
12	28	Medical abortion	Fatal	Uterus	Necrotizing endomyometritis	Positive	Negative	Positive	Positive for C. perfringens
13	40	Postpartum	Fatal	Uterus	Acute endomyometritis	Positive	Negative	Positive	Positive for C. perfringens
14	41	Postpartum	Fatal	Uterus	Necrotizing endometritis	Positive	Negative	Positive	Positive for C. perfringens
15	37	Medical abortion	Fatal	Uterus	Necrotizing endometritis	Negative	Negative	Positive	Positive for C. perfringens
16	26	Postpartum	Fatal	Uterus	Necrotic and hemorrhagic uterus	Negative	Negative	Positive	Positive for C. perfringens
17	41	Spontaneous abortion	Fatal	Uterus	Endomyometritis and sepsis	Positive	Negative	Positive	Positive for C. perfringens
18	32	Postpartum	Fatal	GI	Necrotizing enteritis	Positive	Negative	Positive	Positive for C. perfringens
19	16	Medical abortion	Fatal	Appendix	Acute appendicitis and peritonitis	Positive	Positive	Positive	Positive for both
20	40	No association	Fatal	Cervix	Acute cervicitis and endometritis	Positive	Positive	Positive	Positive for both