Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers
Table 2
Effect of BAP and KIN on shoot multiplication of nodal explants of KPF 4 variety of passion fruit after 4 and 8 weeks of culture.
PGR
Conc. (mg L−1)
4 weeks
8 weeks
Percentage no. of explants with shoots (%)
Mean no. of shoots per explant
Mean no. of leaves
Shoot height (cm)
Percentage no. of explants with shoots (%)
Mean no. of shoots per explant
Mean no. of leaves
Mean shoot height (cm)
BAP
0.0
44.0
0.44 ± 0.1
0.28 ± 0.1
0.04 ± 0.01
55.0
0.61 ± 0.12
0.72 ± 0.18
0.07 ± 0.02
1.0
94.4
0.95 ± 0.1
1.95 ± 0.3
0.16 ± 0.02
100.0
1.0 ± 0.0
3.57 ± 0.31
0.85 ± 0.16
2.0
100.0
1.0 ± 0.0
3.44 ± 0.2
0.68 ± 0.07
100.0
1.06 ± 0.05
5.0 ± 0.23
1.51 ± 0.11
3.0
94.4
2.06 ± 0.3
1.83 ± 0.3
0.22 ± 0.04
100.0
3.83 ± 0.44
3.33 ± 0.37
0.83 ± 0.11
KIN
1.0
94.4
0.94 ± 0.1
1.06 ± 0.2
0.16 ± 0.03
100.0
1.0 ± 0.0
1.72 ± 0.34
0.36 ± 0.08
2.0
100.0
1.0 ± 0.0
1.22 ± 0.2
0.23 ± 0.04
100.0
1.0 ± 0.0
2.28 ± 0.36
0.57 ± 0.09
3.0
100.0
1.0 ± 0.0
2.11 ± 0.3
0.46 ± 0.08
100.0
1.0 ± 0.0
3.61 ± 0.35
0.85 ± 0.10
BAP + KIN
2.0 + 0.5
94.0
4.44 ± 0.8
1.89 ± 0.2
0.36 ± 0.09
100.0
6.39 ± 0.86
3.06 ± 0.30
0.53 ± 0.10
Values represent mean ± standard error of 10 replicates per treatment in three repeated experiments. Means with the same letter within a column are not significantly different at according to Tukey’s Honest significant difference test.
