Mutagenesis and Identification of Sugarcane Mutants Using Survival on Polyethylene Glycol and Leaf Damage under Managed Water Stress

Table 1

Effect of culture medium on callus induction, characterization, and growth after culture for 4 weeks.

MS basal medium supplemented with			Callus formation (%)	Callus characterization		Diameter of callus (cm)
2,4-D (mg/l)	BAP (mg/l)	Coconut water (%)		Color	Texture
3.00	0.50	0.00	9.47^c	Brown	Watery	0.33^b
3.00	0.00	15.00	74.07^b	Yellow or white	Friable and compact	0.80^a
3.00	0.00	0.00	96.43^a	Yellowish	Compact	0.30^b

Means with different letters within a column indicate significant differences () according to the least significant difference test (LSD).