Mutagenesis and Identification of Sugarcane Mutants Using Survival on Polyethylene Glycol and Leaf Damage under Managed Water Stress

Table 2

Influence of ethyl methanesulfonate (EMS) mutagenesis conditions on survival rate and friable callus development.

Treatment (T)	EMS concentrations (mM)	Duration of EMS treatment (h)	Friable callus development	Survival rate of callus (%)
T1	0	0	>75%	100.00^a
T2	10	2	51–75%	100.00^a
T3	10	4	25–50%	77.50^ab
T4	16	4	<25%	57.50^b

Means with different letters within a column indicate significant differences () according to LSD. T1: non-EMS-treated calli, T2: calli treated with 10 mM EMS for 2 h, T3: calli treated with 10 mM EMS for 4 h, and T4: calli treated with 16 mM EMS for 4 h.