Mutagenesis and Identification of Sugarcane Mutants Using Survival on Polyethylene Glycol and Leaf Damage under Managed Water Stress

Table 3

Growth and development evaluation of ethyl methanesulfonate (EMS) treated calli on selective medium supplemented with 15% or 20% PEG for 4 weeks.

EMS concentration (mM)		Selective medium supplemented with 15% PEG			Selective medium supplemented with 20% PEG
Duration for EMS treatment (h)		Initial weight (g)	Weight of callus after evaluation (g)	Weight loss (%)	Initial weight (g)	Weight of callus after evaluation (g)	Weight loss (%)
0	0	2.00	0.27^c	86.50%^a	2.00	0.08^b	96.0%^a
10	2	2.00	1.13^a	43.50%^c	2.00	0.52^a	74.0%^b
10	4	2.00	0.87^ab	56.50%^c	2.00	0.26^a	87.0%^b
16	4	2.00	0.58^bc	71.00%^b	2.00	0.33^a	83.5%^b
F-test

Means with different letters within a column indicate significant differences according to LSD. significant at , respectively.