Mutagenesis and Identification of Sugarcane Mutants Using Survival on Polyethylene Glycol and Leaf Damage under Managed Water Stress

Table 4

Regeneration rate and development of mutant sugarcane plantlets on regeneration media and survival rate of mutant sugarcane plantlets on selective medium with 20% PEG.

EMS concentration (mM)	Duration of EMS treatment (h)	No. of shoots/callus	Shoot regeneration rate (%)	Average number of plantlets	Average plantlet height (cm)	Average number of roots (cm)	Root length (cm)	Plantlet survival rate on MS + 20% PEG (%)
0	0	2.64	70.63	18.00^b	5.47	0.88^b	1.24	39.78^b
10	2	2.76	88.75	25.99^b	5.46	1.55^b	1.57	72.25^a
10	4	1.93	85.63	38.33^ab	5.39	1.37^b	1.45	75.85^a
16	4	2.98	95.63	50.13^a	5.24	3.60^a	1.23	60.61^a
F-test		NS	NS		NS		NS

Means with different letters within a column indicate significant differences according to LSD. non-significant or significant at , respectively.