Research Article
Molecular Determination of Toxigenic Potential of Fusarium spp. Isolated from Seeds of Wheat (Triticum aestivum) Genotypes and Evaluation of Levels of Fumonisins in the Grains at Harvest in Three Major Wheat Producing Counties in Kenya
Figure 2
Agarose 1% gels electrophoresis showing PCR products of the DNA for Fusarium spp. isolates obtained with the universal primers ITS1R and ITS4F. (a) Isolates’ Labels: 8- PT8, 9- PT9, 10- PT10, 11- PT11, 12- PT12, 13- PT53 and 14- PT44. The ladder is 1kb. Primers used are: ITS1R and ITS4F. The band for positive result is at 500–550bp. (b) Isolates’ Labels: PT2, PT19, PT25, PT27, PT28, PT36, PT38, PT39 and PT40. Primers used: ITS1 R and ITS4 F. The ladder is 1Kb O Gene-ruler. Expected band is between 500–550 bp. (c) Isolates’ labels: PT74, PT70, PT89, PT180, PT191, PT192, PT194, PT193, PT195, PT195a, PT46, PT14, and PT24. Primers used: ITS1 R and ITS4 F. The ladder is 1 Kb O Gene-ruler. Expected band is between 500 bp and 550 bp.
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