Investigating Rice Blast Resistance Gene Distribution among Landrace Rice Varieties in Lower Northern Thailand for Improving Rice Cultivars

Table 2

Specific primers for blast resistance gene investigation.

R Genes	Primer sequence (5′⟶3′)	Tm (°C)	Amplicon size (bp)	References
Pi9	F; 5′ CCC​AAT​CTC​CAA​TGA​CCC​ATA​AC 3′	57	466	[15]
	R; 5′ CC GAC​TAA​GTA​CTG​GCT​TCG​ATA 3′
Pib	F; 5′ GAA​CAA​TGC​CCA​AAC​TTG​AGA 3′	50	365	[17]
	R; 5′ GGG​TCC​ACA​TGT​CAG​TGA​GC 3′
Pi-ta	F; 5′ AGC​AGG​TTA​TAA​GCT​AGG​CC 3′	52	1,042	[18]
	R; 5′ CTA​CCA​ACA​AGT​TCA​TCA​AA 3′