Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation

Figure 3

Subcellular localization of the identified proteins based on GO analysis. (a) 881 proteins identified in DOCΔX samples; (b) 659 proteins identified in SDSΔX samples generated using STRAP. The identified subcellular localization profiles are largely similar between both samples, with only the DOCΔX samples having proteins located in the endosome. The larger number of proteins identified from the DOCΔX samples also showed more even distribution of proteins across all subcellular locations. Percentages are rounded to the nearest whole number.

(a)

(b)