Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

Figure 4

Effect of the concentration of sodium dihydrogen phosphate. Mobile phase: aqueous buffer/methanol/acetonitrile of 21 : 3 : 1 (v/v/v), , concentration of sodium dihydrogen phosphate in aqueous buffer of 0, 5, 8, and 12 mM, 12 mM SBE-β-CD, pH of 2.5, flow rate of 1.0 mL/min, detection wavelength of 240 nm, temperature of 25°C, and , and retention factors of ()-CIT and ()-CIT, respectively; , resolution.