The Development of DNA Based Methods for the Reliable and Efficient Identification of<i> Nicotiana tabacum</i> in Tobacco and Its Derived Products

<div>Detection of tobacco components in different tobaccocultivars. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method.<i> Lane 1</i>: NTC;<i> lane 2</i>: PTC;<i> lanes 3–17</i>: 15 tobacco samples of different cured tobacco varieties;<i> lanes 18-19</i>: two fresh tobacco samples;<i> lane M</i>: Trans 2K DNA marker. Ct was expressed as mean Ct <svg height="7.35473pt" id="M4" style="vertical-align:-0.3499303pt" version="1.1" viewbox="-0.0498162 -7.0048 7.75925 7.35473" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M535 290V340H323V533H265V340H52V290H265V84H323V290H535ZM535 -22V28H52V-22H535Z" id="g117-37"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g></svg> SD from 3 independent experiments with three replications.</div>

International Journal of Analytical Chemistry

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Figure 3

Figure 3: The Development of DNA Based Methods for the Reliable and Efficient Identification of<i> Nicotiana tabacum</i> in Tobacco and Its Derived Products 

Figure 3 | The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products 