Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses

Figure 4

Effect of solvents on plant hormone extraction. Concentration of endogenous plant hormones (ZA, ABA, SA, JA, and BR) which were extracted by eight different solvents [P-100% isopropanol, M-100% methanol, PW (75 : 25, isopropanol : water), MW (75 : 25, methanol : water), PWA (75 : 24 : 1, isopropanol : water : acetic acid), MWA (75 : 24 : 1, methanol : water : acetic acid), PA (99 : 1, isopropanol : acetic acid), and MA (99 : 1, methanol : acetic acid)] from control and drought-stressed A. thaliana rosette leaves. “FW” represents fresh weight of plant sample. Asterisks denote a significant hormonal difference between control and drought-stressed plants and “nd” refers to not detected (, -test, , ).