Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses

Table 2

Overview of validated parameters (, retention time; LOD, limit of detection; LOQ, limit of quantification; RE, recovery in percent; RSD, relative standard deviations; and SSE, signal suppression/enhancement).

Sr. number	Hormone	⁢Coefficient of; determination ()	⁢Precision (% RSD) at		RE (%)	SSE (%)	LOD (ng mL^−1)	LOQ (ng mL^−1)
				Peak area
1	Zeatin	0.989	0.63	10.4	83.8	105.7	0.26	0.80
2	(±)-Abscisic acid	0.984	0.31	6.7	92.7	103.7	0.49	1.48
3	Salicylic acid	0.998	0.14	0.08	93.1	104.0	0.04	0.13
4	(±)-Jasmonic acid	0.999	0.15	5.3	82.6	88.01	0.35	1.05
5	Brassinolide	0.990	0.12	19.9	87.5	96.2	0.29	0.87