Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine

<table class="fixed-width table-group" id="tab3"><tr><td><table class="table"><colgroup><col style="width:7.03em"/><col style="width:6.96em"/><col style="width:6.93em"/><col style="width:7.50em"/></colgroup><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td class="align_left">Analyte</td><td class="align_center">LOQ (<i>μ</i>g/mL)</td><td class="align_center">LOD (<i>μ</i>g/mL)</td><td class="align_center">LLOQ (<i>μ</i>g/mL)</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td class="align_left">Uric acid</td><td class="align_center">0.89</td><td class="align_center">0.30</td><td class="align_center">3.12</td></tr><tr><td class="align_left">Creatinine</td><td class="align_center">1.05</td><td class="align_center">0.35</td><td class="align_center">3.12</td></tr><tr><td class="align_left">Hypoxanthine</td><td class="align_center">0.84</td><td class="align_center">0.28</td><td class="align_center">6.25</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Limit of quantification (LOQ), limit of detection (LOD), and lower limit of quantification (LLOQ) for uric acid, creatinine, and hypoxanthine.</div>

International Journal of Analytical Chemistry

tab3

Table 3

Table 3: Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine 

Table 3 | Development and Validation of a Simple High Performance Liquid Chromatography/UV Method for Simultaneous Determination of Urinary Uric Acid, Hypoxanthine, and Creatinine in Human Urine 