Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

Figure 4

Comparison of glycosylation patterns of the reference rAb and the biosimilar rAb samples, obtained by LC-ESI-MS/MS. The glycosylation patterns for the reference rAb sample were obtained by triplicate analysis and averaged. All datasets acquired from each glycoprotein sample were normalized before comparative analysis.