Indoxyl Acetate as a Substrate for Analysis of Lipase Activity

Table 3

Statistic data of concentration curves of IA in the time range of 0–30 min.

Time (min)	Km (mmol/l)	Vmax (nkat)	R	kcat (s^−1)	LOD (mmol/l)
10	9.27	163	0.999	0.157	0.209
15	9.16	133	0.999	0.127	0.122
20	8.72	113	0.999	0.108	0.102
25	8.12	96.5	0.999	0.093	0.100
30	8.49	90.3	0.999	0.087	0.092

K_m—Michaelis–Menten constant, V_max—maximum velocity, R—correlation coefficient, k_cat—turnover number, LOD—limit of detection. The values belong to the volume 10 μl of lipase (200 U).