A Simple Spectrophotometric Method for Determination of Glyoxylic Acid in Its Synthesis Mixture

Figure 7

Optical absorption versus volume of tryptophan 0.016 M solution in the reagent at 0.25 mL glyoxylic acid 0.028 M, 2 mL ferric chloride 0.025 M, and 5 mL concentrated sulfuric acid.