Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay

Figure 4

Standard Lowry assay carried out in micro-centrifuge tubes. (a) Reagent blank. (b) BSA standards with (i) 100 μg/ml, (ii) 150 μg/ml, (iii) 200 μg/ml, (iv) 250 μg/ml, (v) 300 μg/ml, (vi) 350 μg/ml, (vii) 450 μg/ml, and (viii) 500 μg/ml concentrations. (c) The BSA sample with an unknown concentration. (d) The samples treated with Lowry’s reagent: (i) the blank and (ii) a crude protein extract of Leishmania parasites.