Quantification of Soluble or Insoluble Fractions of Leishmania Parasite Proteins in Microvolume Applications: A Simplification to Standard Lowry Assay

Table 2

Sensitivity ranges of most established protein assay methods.

Protein assay method	Sensitivity range
Biuret method [6]	1–10 mg
Hartree’s modified Lowry assay [7]	15–110 μg
Bradford or Coomassie brilliant blue method [13]	1–200 μg
Colloidal gold method [18]	20–640 ng
Bicinchoninic acid assay (Smith or BCA method) [15]	0.2–50 μg
Lowry protein assay [11]	2–100 μg
The proposed method	1–50 μg