Efficient β-Carboline Alkaloid-Based Probe for Highly Sensitive Imaging of Endogenous Glutathione in Wheat Germ Tissues

Figure 4

Microscopic images of HeLa cells. (a) Blank control. (b) Corresponding bright field image. (c) Preincubation with 5 mM NEM thiol scavenger for 1 h at 37°C, followed by incubation with 40 μM KL-DN for 3 h at 37°C. (d) Corresponding bright field image. (e) Incubation for 3 h at 37°C with 40 μM KL-DN. (f) Corresponding bright field image. Scale bar = 200 μm.