Rapid Determination of Pemetrexed Concentration and Distribution in Human Breast Cancer Cells (McF-7) Based on UHPLC-MS/MS

Table 6

The concentration variations of pemetrexed in medium, cytoplasm, and cell nucleus (n = 3, mean ± SD).

Time (h)	Intracellular sample concentration (ng/mL)	Nuclear sample concentration (ng/mL)	Medium sample concentration (ng/mL)
0	ND	ND	ND
0.5	4.24 ± 0.53	3.60 ± 0.04	1689.69 ± 83.79
1	7.45 ± 2.53	4.25 ± 0.01	1793.39 ± 18.75
2	19.94 ± 1.26	6.52 ± 0.34	1742.55 ± 24.40
3	41.2 ± 0.76	4.98 ± 1.87	1741.00 ± 42.47
6	35.32 ± 0.64	29.10 ± 0.66	1468.20 ± 43.84

ND: not detected.