Research Article
Rapid Quantification of Chlorpromazine Residues in Pork Using Nanosphere-Based Time-Resolved Fluorescence Immunoassay Analyzer
Table 3
Comparison of nano-TRFIA and UPLC-MS/MS for CPZ detection in pork (n = 5).
| | Spiked concentration (μg/kg) | UPLC-MS/MS | Nano-TRFIA | | Detected concentration (μg/kg) | Recovery (%) | RSD (%) | Detected concentration (μg/kg) | Recovery (%) | RSD (%) |
| | 1.0 | 0.96 ± 0.03 | 96.40 | 3.99 | 0.93 ± 0.05 | 92.60 | 5.59 | | 2.0 | 1.92 ± 0.04 | 96.20 | 2.25 | 1.92 ± 0.04 | 95.80 | 2.11 | | 4.0 | 3.92 ± 0.09 | 98.00 | 2.63 | 3.89 ± 0.12 | 97.15 | 3.59 | | 6.0 | 5.98 ± 0.10 | 99.73 | 1.80 | 6.04 ± 0.13 | 100.63 | 2.40 | | 8.0 | 8.03 ± 0.16 | 100.35 | 2.23 | 8.09 ± 0.15 | 101.15 | 2.09 | | 10.0 | 10.06 ± 0.14 | 100.58 | 1.56 | 10.47 ± 0.45 | 104.70 | 4.77 |
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CPZ, chlorpromazine; nano-TRFIA, nanosphere-based time-resolved fluorescence immunoassay; RSD, relative standard deviation; UPLC-MS/MS, ultraperformance liquid chromatography-tandem mass spectrometry.
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