Development and Validation of a LC-MS/MS Method for the Determination of Nitrofuran Metabolites in Soft-Shell Turtle Powder Health Food Supplement

Figure 2

Change in pH over time and amount of 2-NP-AOZ detected in the incurred sample. (a) Concentration of detected 2-NP-AOZ in samples treated with 10, 20, 30, and 50 mL of 0.125 M HCl at 37°C. The results of the experiments with 50 mL were discarded because of errors. (b) Concentration of detected 2-NP-AOZ using 10, 12, 13, 14, 15, and 20 mL of 0.5 M HCl at 45°C. Line graph: pH value over time (unit: pH). Bar graph: final detected concentration of 2-NP-AOZ in the incurred sample under each test condition (unit: μg kg⁻¹).