Research Article
Development and Validation of a LC-MS/MS Method for the Determination of Nitrofuran Metabolites in Soft-Shell Turtle Powder Health Food Supplement
Table 2
Calibration curves, sensitivity, corrected intraday and interday accuracies, and precision for samples spiked with working standard solution (n = 6).
| | Target analyte | Linearity range (μg kg−1) | Spiked level (μg kg−1) | Calibration curve equation (r) | Intraday series (% RSD) | Interday series (% RSD) | LOD (μg kg−1) | LOQ (μg kg−1) | CCα (μg kg−1) | CCβ (μg kg−1) |
| | 2-NP-AHD | 0.2–20 | 0.5 | y = 0.0124x + 0.1991 (0.99969) | 98.8 (3.2) | 92.8 (3.6) | 0.07 | 0.23 | 0.25 | 0.30 | | 1.0 | 96.9 (3.2) | 101.3 (3.8) | | 2.0 | 102.5 (2.1) | 98.3 (2.9) | | 10.0 | 98.7 (1.7) | 99.4 (2.5) |
| | 2-NP-SEM | 0.2–20 | 0.5 | y = 0.0477x + 0.1280 (0.99971) | 95.5 (3.8) | 92.6 (4.5) | 0.05 | 0.17 | 0.18 | 0.30 | | 1.0 | 92.5 (3.1) | 98.2 (3.5) | | 2.0 | 94.8 (2.1) | 92.7 (3.8) | | 10.0 | 95.2 (2.2) | 93.9 (2.8) |
| | 2-NP-AOZ | 0.3–20 | 0.5 | y = 0.0127x + 0.2459 (0.99941) | 82.2 (3.7) | 85.2 (4.8) | 0.09 | 0.29 | 0.30 | 0.34 | | 1.0 | 84.7 (3.2) | 88.5 (3.4) | | 2.0 | 87.5 (2.4) | 108.1 (2.8) | | 10.0 | 90.4 (2.8) | 87.6 (3.1) |
| | 2-NP-AMOZ | 0.2–20 | 0.5 | y = 0.0235x + 0.2192 (0.99980) | 92.6 (2.2) | 90.4 (3.1) | 0.05 | 0.17 | 0.27 | 0.32 | | 1.0 | 85.6 (3.4) | 93.3 (3.7) | | 2.0 | 93.0 (2.3) | 94.8 (3.4) | | 10.0 | 95.6 (1.5) | 96.5 (2.2) |
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