Formulation and development of Serratiopeptidase enteric coated tablets and analytical method validation by UV Spectroscopy

Table 1

Composition of formulated tablets.

S. No.	Materials (mg/tablet)	F1	F2	F3	F4	F5
1.	Serratiopeptidase	10.50	10.50	10.50	10.50	10.50
2.	MCCP pH 101	141.00	—	—	—	—
3.	MCCP pH 102	—	139.50	132.50	132.50	132.50
4.	Colloidal silicon dioxide	2.00	2.50	4.00	4.00	4.00
5.	Sodium starch glycolate	3.50	4.50	5.50	5.50	5.50
6.	Crospovidone	—	—	4.00	4.00	4.00
7.	Magnesium stearate	1.50	1.50	2.00	2.00	2.00
8.	Stearic acid	1.50	1.50	1.50	1.50	1.50
Total weight (uncoated)		160.00	160.00	160.00	160.00	160.00
Seal coating materials
1.	White shellac	—	—	5.10	5.70	6.37
2.	Castor oil	—	—	0.062	0.062	0.062
3.	Isopropyl alcohol	—	—	105.00	105.00	105.00
Enteric coating materials
1.	HPMC phthalate 40	—	—	8.00	8.00	13.44
2.	Ethyl cellulose	—	—	2.74	2.74	3.820
3.	Castor oil	—	—	0.47	0.47	0.97
4.	Titanium dioxide	—	—	0.55	0.55	0.55
5.	Lake Erythrosine	—	—	0.11	0.11	0.11
6.	Isopropyl alcohol	—	—	85.00	85.00	85.00
7.	Methylene chloride	—	—	150.00	150.00	150.00
Targeted weight in mg (coated)		—	—	170.00	170.00	170.00