Simultaneous Determination of 25 Ginsenosides by UPLC-HRMS via Quantitative Analysis of Multicomponents by Single Marker

Table 1

Calibration curves, limit of quantification (LOQ), and limit of detection (LOD) of UPLC-MS methods for determination of 25 ginsenosides.

Ginsenoside	RT	Calibration curve	Test range (ug/mL)	r^2	LOQ (ng/mL)	LOD (ng/mL)
Rg1	1.46	Y = 64984X + 980287	0.02-2	0.9990	0.106	0.032
Re	1.46	Y = 41225.1X + 688583	0.02-2	0.9959	0.164	0.049
Rf	4.08	Y = 42146.9X − 44017.1	0.01-0.5	0.9994	0.116	0.035
Rh1	5.02	Y = 238413X − 153145	0.002-0.2	0.9994	0.025	0.007
Rb1	5.44	Y = 6240.95X + 85602.4	0.02-2	0.9964	1.163	0.349
Rg2	5.08	Y = 52298.5X − 612776	0.05-1	0.9924	0.012	0.003
Rc	6.09	Y = 22275X − 24796.1	0.01-1	0.9991	0.182	0.055
F1	6.73	Y = 292355X − 175557	0.002-0.2	0.9995	0.029	0.009
Rb2	7.13	Y = 20778.7X − 36514.3	0.01-1	0.9994	0.313	0.094
Rb3	7.59	Y = 20695.1X + 48835.8	0.05-2	0.9994	0.513	0.154
Rd	9.25	Y = 61029.8X − 558838	0.1-2	0.9989	0.015	0.005
G17	10.07	Y = 85248.5X − 57819.7	0.002-0.2	0.9998	0.217	0.065
nFe	10.8	Y = 29919.9X − 7299.02	0.002-0.2	0.9996	0.192	0.058
CO	11.61	Y = 31396.7X − 13591.7	0.001-0.2	0.9990	0.217	0.065
nFd	12.01	Y = 20835.8X − 25094.8	0.01-0.2	0.9959	0.833	0.250
F2	13.68	Y = 212189X − 115446	0.002-0.2	0.9998	0.029	0.009
G75	15.63	Y = 231218X − 99660.6	0.001-0.2	0.9991	0.042	0.013
Rg3	15.89	Y = 149379X − 82845.1	0.002-0.2	0.9993	0.154	0.046
PPT	14.75	Y = 27249.2X − 50845.3	0.002-0.05	0.9984	0.150	0.045
Mc	17.29	Y = 58309.3X − 14125.5	0.001-0.2	0.9995	0.294	0.088
CY	18.02	Y = 41233.6X + 36194	0.001-0.2	0.9993	0.175	0.053
CMx	18.46	Y = 80902.7X − 22662.5	0.002-0.2	0.9990	0.189	0.057
CK	19.31	Y = 140955X + 96724.4	0.001-0.2	0.9998	0.018	0.005
Rh2	19.6	Y = 316346X − 188172	0.002-0.2	0.9996	0.016	0.005
PPD	20.99	Y = 3099.89X − 4843.21	0.002-0.2	0.9990	0.535	0.160