Simultaneous Determination of 25 Ginsenosides by UPLC-HRMS via Quantitative Analysis of Multicomponents by Single Marker

Table 5

Effects of column temperature and flow rate upon relative response factor (RCF) and relative retention time (RT_R).

	Effects of column temperature				Effects of flow rate
	RCF		RTR		RCF		RTR
Ginsenoside	Mean	RSD%	Mean	RSD%	Mean	RSD%	Mean	RSD%
Rg1	0.29	3.45	0.21	0.00	0.30	3.33	0.22	5.33
Re	0.47	2.44	0.21	0.00	0.49	2.37	0.22	4.55
Rf	0.48	1.21	0.56	1.79	0.48	1.19	0.58	1.00
Rb1	3.08	3.83	0.77	0.75	3.19	1.58	0.76	2.02
Rg2	0.34	4.45	0.70	1.43	0.35	1.63	0.72	0.81
Rh1	0.09	3.94	0.70	1.66	0.09	3.32	0.71	0.00
Rc	0.91	3.34	0.86	0.67	0.92	0.00	0.85	1.18
F1	0.08	0.00	0.95	0.61	0.08	0.00	0.96	1.04
Rb3	0.98	3.11	1.06	0.00	0.99	1.01	1.07	0.54
Rd	0.32	3.13	1.29	0.45	0.33	1.73	1.24	5.26
GXVII	0.25	4.00	1.41	0.41	0.25	2.28	1.36	4.80
nFe	0.67	1.49	1.52	0.38	0.68	0.85	1.46	4.12
CO	0.64	2.40	1.64	0.71	0.63	2.44	1.58	3.16
nFd	1.06	1.44	1.69	0.34	1.05	1.45	1.64	3.37
F2	0.10	5.97	1.92	0.52	0.10	0.00	1.95	6.48
G75	0.09	3.27	2.19	0.00	0.09	4.61	2.12	3.55
Rg3	0.13	4.56	2.21	0.90	0.13	0.00	2.16	3.50
PPT	1.03	2.03	2.05	1.49	1.02	1.96	1.96	5.61
Mc	0.34	2.94	2.43	0.24	0.35	1.67	2.33	4.54
CY	0.45	4.44	2.53	0.40	0.45	3.37	2.41	6.08
CMx	0.26	3.85	2.59	1.68	0.28	5.52	2.45	7.54
CK	0.11	5.41	2.69	1.14	0.10	5.59	2.53	7.50
Rh2	0.07	5.09	2.72	1.18	0.07	3.36	2.57	7.60
PPD	6.76	1.78	2.90	1.43	6.73	1.43	2.75	7.46