Research Article

Chemical Identification and Antioxidant Screening of Bufei Yishen Formula using an Offline DPPH Ultrahigh-Performance Liquid Chromatography Q-Extractive Orbitrap MS/MS

Table 3

Candidate antioxidants identified from BYF and rat serum after the oral administration of BYF (10 mM DPPH).

CompoundIntensity reducedRat serum

21Calycosin-7-O-glu or its isomer (a)−100 ± 0%
36Diosmetin-6-C-glu/pratensein-7-O-glu (b)−100 ± 0%
53N-E-feruloyl tyramine−100 ± 0%+
104Chrysoeriol−100 ± 0%+
28Diosmetin-6-C-glu/pratensein-7-O-glu (a)−100 ± 0%
30Diosmin−100 ± 0%
26Rosmarinic acid−100 ± 0%
66Sudachiin B/C−100 ± 0%
32Tetrahydroxy-dimethoxyflavone (a)−100 ± 0%
112Tetrahydroxy-dimethoxyflavone (b)−100 ± 0%
109Tetrahydroxy-trimethoxyflavone−100 ± 0%
181Tetramethoxyflavanone−100 ± 0%
107Trihydroxy-trimethoxyflavone−100 ± 0%
172Schisanhenol−99.88 ± 0.11%+
58Calycosin−99.19 ± 0.17%+
39Hesperetin-7-O-glu or its isomer (b)−99.14 ± 0.86%
170Gomisin L1 or its isomer (c)−99.09 ± 0.79%
24Hesperetin-7-O-glu or its isomer (a)−98.87 ± 1.11%+
22Hesperidin−97.92 ± 1.87%+
127Neoicariin/wushanicariin/icariside I or their isomer (e)−97.78 ± 0.2%
38Hesperetin−97.23 ± 2.55%+
180Gomisin L1 or its isomer (e)−95.7 ± 3.77%
83Epimedoside C−93.81 ± 0.8%+
176Gomisin L1 or its isomer (d)−91.19 ± 7.72%
142Rosmarinic acid methylester−87.71 ± 1.01%
163Natsudaidai−77.87 ± 20.02%
161Gomisin L1 or its isomer (b)−72.78 ± 0.21%
167Gomisin K1−66.94 ± 4.97%+
119Pentamethoxyflavanone (a)−60.01 ± 35.31%
136Gomisin L1 or its isomer (a)−56.9 ± 3.72%+
95Naringenin−52.81 ± 2.96%+
179Schisantherin C (angeloylgomisin P) or its isomer (c)−52.03 ± 5.84%
114Anhydroicaritin-3-O-rhamnosyl-7-O-glucopyranoside/sagittatoside A−48.26 ± 1.63%+
98Epimedokoreanoside II/sagittatoside C−47.47 ± 14.34%
120Anhydroicaritin or its isomer (c)−46.99 ± 3.58%
99Ikarisoside F−46.02 ± 5.68%
121Sagittatoside B−45.64 ± 2.03%+
149Ginsenoside Rg5/Rk1−44.74 ± 0.63%
110Baohuoside II−44.32 ± 4.95%
1222″-O-rhamnosylicariside II/anhydroicaritin 3-O-2″-rha-rha−43.86 ± 3.13%+
133Icariside II (baohuside I)−41.97 ± 1.9%+
132Anhydroicaritin or its isomer (d)−40.5 ± 0.89%
97Apigenin−39.92 ± 14.63%+
113Anhydroicaritin or its isomer (b)−39.57 ± 3.19%
92Astragaloside Iv−39.11 ± 5.35%
93Epimedokoreanoside I−35.72 ± 8.33%
177Baohuosu−35.12 ± 2.68%+
77Icaritin-3-O-rha−32.89 ± 6.35%
117Formononetin−32.3 ± 1.23%+
124Meranzin/isomeramazin (b)−31.73 ± 6.19%
80Ginsenoside Rb1−31.42 ± 7.33%
17813-hydroxy-9,11-octadecadienoic acid or its isomer (c)−31.09 ± 10.58%
75Naringenin isomer (b)−30.76 ± 0.8%
129Hexamethoxyflavone (a)−28.6 ± 3.48%+
150Schisandrol B/epigomisin O−26.94 ± 2.99%+
33Apigenin-7-O-gluA−26.88 ± 8.86%+
134Sinensetin−26.76 ± 1.67%+
94Benzoylpaeoniflorin/paeonivayin or their isomer (a)−26.62 ± 4.87%
91Ebeiedinone/delavinone/zhebeirine (puqiedinone) (a)−26.21 ± 1.23%+
65Peimisine isomer (e)−25.88 ± 5.23%
139Pentamethoxyflavanone (b)−25.33 ± 4.29%
1473,5,6,7,8,3′,4′-Heptamethoxyflavone−25.31 ± 3.13%+
81Sempervirenoside B−24.59 ± 11.39%
151Tangeretin−24.37 ± 2.2%+
126Isosinensetin−24.31 ± 1.4%+
143Nobiletin−23.76 ± 0.63%+
160Angeloylgomisin H or its isomer (b)−23.74 ± 3.76%
154Angeloylgomisin H or its isomer (a)−23.58 ± 3.47%+
108Astragaloside II/isoastragaloside II−22.98 ± 5.24%
116Soyasaponin I−22.79 ± 7.26%+
189Schisandrin C−22.79 ± 11.89%+
100Ebeiedinone/delavinone/zhebeirine (puqiedinone) (b)−22.71 ± 4.35%
82Melitidin−22.65 ± 6.9%+
44Peimisine nitrogen oxide−22.07 ± 3.99%
155Schisandrin B (γ-schisandrin) isomer (a)−21.68 ± 1.42%+
153Hexamethoxyflavone (b)−21.31 ± 3.14%
43Peimisine isomer (c)−21.14 ± 10.74%
165Benzoyl gomisin H−20.24 ± 0.72%
71Astragaloside V/VI/VII−20.14 ± 21.7%