Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Table 3

Stability of lamotrigine, oxcarbazepine, lacosamide, and topiramate in rat plasma under various storage conditions (n = 3).

Compound	Concentration (ng/mL)	Autosampler (4°C, 12 h)		Ambient (2 h)		–20°C (30 d)		Freeze-thaw
		Accuracy	RSD	Accuracy	RSD	Accuracy	RSD	Accuracy	RSD
	10	97.2	4.7	107.9	5.1	96.7	10.1	106.7	8.9
Lamotrigine	900	105.1	4.8	100.0	5.4	93.1	11.6	95.3	10.4
	4500	99.9	3.0	95.2	2.7	101.3	2.8	107.2	3.8
	10	102.8	3.4	103.4	7.9	94.2	10.1	93.9	12.8
Oxcarbazepine	900	105.8	9.5	101.0	7.9	105.6	6.0	102.4	8.3
	4500	97.0	8.7	101.1	5.1	94.1	8.4	93.1	7.3
	10	102.2	3.2	99.2	3.1	94.9	9.1	97.1	10.5
Lacosamide	900	99.8	3.4	99.1	2.3	95.5	4.4	100.5	5.0
	4500	97.8	4.5	95.2	2.6	101.4	5.9	98.6	5.3
	10	96.4	7.5	96.3	9.2	92.9	9.6	97.2	11.7
Topiramate	900	98.0	8.9	103.5	5.5	104.0	7.0	102.5	8.4
	4500	105.1	4.9	95.2	9.8	94.8	3.4	90.4	10.2