Determination of Senegenin and Tenuifolin in Mouse Blood by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry and Their Pharmacokinetics

Table 2

Stability of senegenin and tenuifolin in mouse blood.

Compound	Concentration (ng/mL)	Autosampler (4°C, 12 h)		Ambient (2 h)		−20°C (30 d)		Freeze-thaw
		Accuracy	RSD	Accuracy	RSD	Accuracy	RSD	Accuracy	RSD
Senegenin	10	98.0	3.6	98.5	3.4	93.1	10.3	88.7	11.9
	450	100.6	7.3	107.7	8.3	106.6	10.0	106.2	11.5
	3500	102.2	3.0	97.9	1.5	103.8	8.0	99.3	8.1
Tenuifolin	10	101.8	3.3	99.5	4.1	108.3	11.3	90.0	11.4
	450	102.6	6.8	103.8	2.6	94.6	3.7	104.7	4.4
	3500	97.8	2.5	98.5	5.0	104.1	10.4	90.3	10.4