Evaluation of Alternative Transport Media for RT-qPCR-Based SARS-CoV-2 Testing

Figure 3

Comparison of the Ct values (determined by real-time PCR) for the three SARS-CoV-2 genes in the 46 NPS samples that were positive or negative for the virus. Samples were diluted in GeneTM, eNAT™, or PBS. Comparison of the (a) S gene, (b) RdRP gene, and (c) N gene in NPS samples diluted in GeneTM and eNAT™. Comparison of the (d) S gene, (e) RdRP gene, and (f) N gene in NPS samples diluted in GeneTM and PBS. Two replicates per sample were assayed using real-time PCR under the indicated conditions. Ct, cycle threshold; S gene, spike gene; RdRP gene, RNA-dependent RNA polymerase gene; N gene, nucleocapsid gene.

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