Evaluation of Alternative Transport Media for RT-qPCR-Based SARS-CoV-2 Testing

Figure 4

Stability of the SARS-CoV-2 (a, d) S gene, (b, e) RdRP gene, and (c, f) N gene following dilution of the NPS samples in CTM in GeneTM, eNAT™, or PBS at (a–c) cold temperature (4°C) and (d–f) room temperature (25°C). Six replicates per sample were assayed by real-time PCR under each of the indicated conditions. The dotted line indicated the cut-off value of ±3 Ct, below which samples were deemed stable. Ct, cycle threshold; S gene, spike gene; RdRP gene, RNA-dependent RNA polymerase gene; N gene, nucleocapsid gene.

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