Determination of Gypenoside A and Gypenoside XLIX in Rat Plasma by UPLC-MS/MS and Applied to the Pharmacokinetics and Bioavailability

Table 2

Stability of gypenoside A and gypenoside XLIX in rat plasma (%).

Compound	Concentration (ng/mL)	Autosampler (4°C, 12 h)		Ambient (2 h)		−20°C (30 d)		Freeze-thaw
		Accuracy	RSD	Accuracy	RSD	Accuracy	RSD	Accuracy	RSD
Gypenoside A	5	101.9	7.8	93.7	9.2	102.7	13.3	110.3	14.8
	250	100.5	2.8	99.9	5.3	93.8	7.2	108.6	7.2
	2500	96.6	5.6	106.0	5.6	99.8	5.9	92.2	6.0
	5	101.1	11.7	107.7	11.5	112.2	14.5	91.2	13.3
Gypenoside XLIX	250	108.6	8.4	97.4	6.5	90.7	7.9	87.9	13.0
	2500	99.5	8.0	95.5	8.8	88.0	1.4	103.9	9.5