Review Article

Review on Characterization, Properties, and Analytical Methods of Cefepime

Table 1

Detection of CP quantitatively by HPLC methods.

Column (C)Mobile phaseFlow rate (ml/min)Wavelength (nm)MatricesReference

Supelcosil ABZ + (5 μm;150 × 4.6 mm)20 mM PDPB pH 2:ACN (94 : 6, , ).1263Human serum[30]
Hypersil BDS C18Acetate buffer pH 4:ACN (97.2 : 2.8 )1254Human plasma[31]
Hypersil BDS C18MeOH:25 mM SDPM pH 3 (87 : 13 )1270Plasma and vitreous fluid[32]
RP-C18Acetate buffer pH 3.5 : MeOH/triethylamine (82 : 18 v/v)1263Goat plasma and milk[33]
RP Ultrasphere XL-ODS C (75 × 4.6 mm I.D.)20 mM AA pH 4 : 7% ACN1254Human serum[34]
Onyx Monolithic C18 (20 cm– 4.6 mm) coupled to Phenomenex C18 GC (5 cm 4.6 mm)MeOH: 10 mM DPHP pH 7 (gradient)1254Human plasma and dialysate[35]
C18 with pre-CNaOH buffer pH 3 : 1 M phosphoric acid: 0.01 Mn-octylamine pH 3.0 : ACN1.3259Human urine[36]
μBondapak C18 (30 cm × 3.9 mm × 10 μm)ACN: acetate buffer (5 : 95 )2280Plasma and dialysate-ultrafiltrate from patients[37]
SupelcosilTMLC-18 (25 cm × 4.6 mm × 5 μm), with a C18 GCACN: 0.075 M acetate buffer pH 5 (8 : 92, )0.8230Human plasma of burn patients[38]
100 × 4.6 mm i.d. Perkin Elmer phenyl C (5 µm)ACN (including 0.015 M pentane sulfonic acid sodium pH 3.4 + glacial acetic acid and 4 with 45% KOH):water (5.5 : 94 )1.5280Aqueous solution[39]
XTerra C18 (250 × 4.6 mm, 5 μm) supported by Phenomenex C18 GC (4 × 3.0 mm)MeOH : 40 mM phosphate buffer pH 3.21260Plasma and amniotic fluid[40]
LiChrospher 100 RP C18 (250 mm × 4 mm, 5 lm particles)MeOH: 10 mM phosphate buffer pH 7 (25 : 75 )1256Human serum, cerebrospinal fluid, and urine[41]
C18Acetate buffer pH 5.1: ACN: MeOH (5 : 20 : 75 v/v)1212Pure and pharmaceutical dosage forms[42]
Phenomenex ODS (4.6 × 250 mm, 5µ)ACN : AA pH 4.9 : (8 : 92)1.5256Pharmaceutical formulations[43]
Acclaim 120 C18 (250 × 4.6 mm, 5 μm particle size)MeOH: sodium acetate buffer pH 6 (11 : 89 )1.8220Injections[44]
Luna C18 (250 × 4.6 mm; 5 μm)Ethanol : water (45 : 55 v)0.5258Pharmaceutical dosage Form[45]
C18 (250 × 25 mm) 25 μmWater: ACN (90 : 10 v/v)1212CP in injections[46]
Princeton-100 C18 (4.6 mm i.d × 250 mm., 5 µn)ACN: 25 mM PDPB pH 6.2 (6 : 9 4 )1210Bulk and pharmaceuticals[47]
Hypersil Gold pentafluorophenyl (PFP) 6 (2.1 by 100 mm, 1.9 m)(a) 10 mM phosphoric acid
(b) ACN
0.5260Human plasma[48]
C18(ACN/0.1 M phosphoric acid/NaOH buffer pH 3): 0.01 M n-octylamine pH 3 (gradient)1256Human urine[49]