|
| Technique | Column (C) | Mobile phase or eluent | Conditions | Matrices | Reference |
|
| R-HPLC-UV + (SPE) | C18 | MeOH : ACN : AA | 257
0.9 | Nutrient admixtures | [61] |
| LC | LiChrosorb RP-C18 (250 × 4.6 mm I.D., 5 μm particle size) | MeOH : mM monosodium phosphoric acid pH 3 (13 : 87 v/v) | 270
1 | The bile duct microdialysis probes | [62] |
| HILIC LC-MS/MS | HPLC Hypersil GOLD C (150 × 4.6 mm, 5 μm)
MS (spray voltage 3500 V, temperature 300°C, vaporizer temperature 400°C) | (a) 0.1% Formic acid:10 mM AA
(b) 0.1% Formic acid:10 mM AA : MeOH (1 : 1 v/v)
(c) 2-Propanol:acetone :ACN (1 : 1: 1 v/v/v) | Gas flow 6.5
Auxiliary gas flow 0.8 | Plasma and cerebrospinal fluid | [63] |
| UPLC-MS/MS | RP Acquity BEH HILIC column (50 mm × 2.1 mm, 1.7 μm, Waters) | (a) (ACN)
(b) 20 mM AFB (Gradient) | 0.5 | Human plasma | [64] |
| (LC-GC-FID) | Extraction solvent (chloroform), SGE capillary C (30m × 0.25 mm) | Water : MeOH (12 : 88 v/v) : NaCl : carbonate/bicarbonate buffer pH 512 | Flow rate 30 Hydrogen gas was used for the FID rate of air which was 300 | NMP in CP (pharmaceutical preparation) | [65] |
| IC-SPE | Metrosep C4 4 mm × 250 mm cation exchange at 30°C | 5% ACN:0.01 mlL−1 nitric acid | 265 | NMP in CP. HCl | [66] |
| SCX-LC/MS/MS | Zorbax300-SCX (2.1 mm × 50 mm, 5 μm) | (a) ACN : 25 mM AFB pH 2.79 (5 : 95 v/v)
(b) ACN : 500 mM AFB pH 2.79 + 25 mM AFB (30 : 70 v/v) | 0.5
Curtain gas flow: 20psi; ion spray voltage: 5500 V; ion gas 1 and 2: | Mouse plasma | [67] |
| LC-MS/MS | Luna HILIC 200A, 100 × 2.0 mm, 3 μm (Phenomenex) with a GC | ACN : 10 mM AFB pH 3.5 (72 : 28 v/v) | 0.3 | Plasma | [68] |
| HPLC-MS/MS | HPLC (Fortis reverse phase C8 (100 mm × 2.1 mm, 3 μm))
MS (voltage 1.5 kV) | (a) Water-formic acid:10 mM AFB (0.1 : 99.9 v/v), B. MeOH | Gas flow rate, 600 l/h | Human serum | [69] |
| VAMS-LC/MS | Phenomenex (2.6 μm, 100 Å, 50 × 4.6 mm) | (a) Water : 5 mM AA pH 5
(b) 5 mM AA in water : ACN (10 : 90 v/v) | 0.5 | Human whole blood | [70] |
| MEKC + UV + LC/MS | PFP Nucleodur HPLC column | MEKC (mM Na2B4O7 + 10 mM Na2HPO4 + 75 mM pH 9.1)
HPLC ((a) 5 mM AFB pH 3, (b) 100% CAN) | 254
0.5 | Urine | [71] |
| HPLC + MS | RP-C18 | ACN : 10 mmolL−1 ammonium acetate (5 : 95) | 0.8 | Raw drug | [72] |
|
