Review on Characterization, Properties, and Analytical Methods of Cefepime

Table 5

Spectrophotometric methods for the analysis of CP.

Technique’s name	Solvent for dissolving and dilution	Conditions	Matrices	Reference
DRIR + XRD	8 ml of acetone was added as an eluent	Spectral limits 3587,3557 cm^−1	Different hydrated forms of CP.2HCl	[73]
FTIR	The samples were diluted to 1000 mg with KBr Potassium bromide was used as a diluent	4000–400 cm^−1	Pharmaceutical formulations	[74]
Savitzky–Golay differentiation filters and Fourier functions	Solutions prepared in concentration 100 μg ml^−1 in water	266	Human plasma	[75]
Complexation with Hg	Solutions were prepared in concentration 20–400 μg ml^−1 in water	257	Pharmaceutical dosage forms	[76]
Spectrophotometry with ammonium molybdate	Solutions were prepared in concentration 1000 μg ml^−1 in water	695	Pharmaceutical dosage forms	[77]
Spectrofluorometry		EXW (307), EMW (297), 435	Dosage forms	[78]
Spectrophotometry using a tetrazolium Salt	Solutions were prepared in concentration 20 μg ml^−1 with MeOH	483	Pharmaceutical dosage forms	[79]
UV spectrometry	Diluted with UB (0.1 M CH3COOH + 0.1 M H3PO4 + 0.1 M H3BO3)	264, 230	Pharmaceutical preparation	[57]
Fluorescence spectroscopy	Solutions were prepared with doubly distilled water	The EW was 280, 295The fluorescent intensity set at 341	Lysosome	[80]
UV + FTIR	Solutions were prepared in water; fluorescence intensity was measured in Tris/HCl solution pH 7.4	EW was 310 and EW set at 435	Pharmaceutical ingredient	[81]
Spectrophotometry	Solutions were prepared and diluted with 0.1 N NaOH	232	Pharmaceutical dosage forms	[82]
Spectrophotometry	Solutions were prepared and diluted with water	570	Pure and pharmaceutical dosage forms	[83]
Derivative spectrophotometry	Solutions were prepared and diluted with water	239, 254	Injections	[84]
Direct-infusion electrospray ionization	The solutions of NMP (N-methyl pyrrolidine) were prepared and diluted with water-MeOH (50 : 50)	ESI (V of 2000 V) flow of 7 l min^−1, GOT of 250°C	NMP in CP	[85]
		FAIMS (V 75 and 375 V), electrode gaps (100 mm) with (700 mm) as a path length
Microbiological assay	Powders were dissolved and diluted in water to give concentrations of 8.0, 16.0, and 32.0 μg m^−1	580	Injectable preparations	[86]