Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Table 3

Results of method optimization.

Column	Mobile phase	Elution mode	Flow rate	Observation	Result
C18	ACN : water (50 : 50, v/v)	Isocratic	1.0 mL/min	Good retention time and peak	Accepted
C18	ACN : water (40 : 60, v/v)	Isocratic	1.0 mL/min	Longer retention time	Rejected
C18	ACN : water (30 : 70, v/v)	Isocratic	1.0 mL/min	Longer retention time	Rejected
C18	ACN : water (60 : 40, v/v)	Isocratic	1.0 mL/min	Peak distortion	Rejected
C18	ACN : water (70 : 30, v/v)	Isocratic	1.0 mL/min	Peak distortion	Rejected