Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Table 7

Results of repeatability and intermediate precision for assay evaluation.

No. of Sample	Sample weight (mg)	Standard area	Sample area	Content of LCZ in cream (% comparing to labeled amount)
Day1, Analyst 1
1	1000.10	5684.624	5708.925	99.97
2	1000.80	5700.509	5700.296	99.75
3	1000.50	5692.440	5710.292	99.95
4	1000.60	5702.616	5704.638	99.84
5	1000.30	5696.776	5708.984	99.95
6	1000.50	5681.942	5706.591	99.89
Average (1–6)				99.89
%RSD (1–6)				0.085
Day2, Analyst 1
7	1000.10	5716.334	5711.201	100.41
8	1000.20	5730.679	5721.259	100.58
9	1000.40	5729.944	5726.216	100.64
10	1000.10	5725.420	5733.249	100.80
11	1000.10	5726.465	5728.945	100.72
12	1000.90	5733.207	5713.012	100.36
Average (7–12)				100.58
%RSD (7–12)				0.171
Day 2, Analyst 2
13	1000.60	5752.034	5711.015	99.81
14	1000.40	5770.969	5710.298	99.82
15	1000.90	5751.785	5716.566	99.88
16	1000.30	5779.596	5721.219	100.02
17	1000.50	5732.576	5708.091	99.77
18	1000.40	5763.467	5706.636	99.75
Average (13–18)				99.84
%RSD (13–18)				0.097