Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Table 9

Robustness data of the applied HPLC method for LCZ.

Parameter	Stability conditions	RT	Avg. peak area	RSD % (peak area)	Tailing factor	Assay%	%RSD (assay)	Number of theoretical plates
Standard solution	0 h	16.97	5629.816	0.159	0.82	—	—	2290.39
	After 24 h at 30°C	17.46	5746.347	0.115	0.80	—	—	2328.47
	After 24 h at refrigerator	17.82	5722.062	0.259	0.81	—	—	2290.36
Sample solution	0 h	17.51	5645.114	0.198	0.83	99.95	0.187	2320.20
	After 24 h at 30°C	17.51	5696.947	0.191	0.82	99.81	0.190	2404.20
	After 24 h at refrigerator	17.28	5674.313	0.406	0.81	99.84	0.409	2266.80