Pharmacokinetic Difference of Six Active Constituents of Huangqi Liuyi Decoction between Control and Diabetic Nephropathy Mouse Models

Table 5

Stability of six analytes in mouse plasma ( ± SD, n = 6).

Analyte	Concertation of analyte (ng/mL)	Sampler 4 h		−20°C 48 h		Three freeze-thaw
		Mean ± SD (ng/mL)	RSD (%)	Mean ± SD (ng/mL)	RSD (%)	Mean ± SD (ng/mL)	RSD (%)
Astragaloside IV	16.02	15.76 ± 1.34	8.52	15.17 ± 1.08	7.10	15.58 ± 1.58	10.14
	801	826.8 ± 49.36	5.97	841.1 ± 54.76	6.51	760.6 ± 50.89	6.69
	1602	1591 ± 113.94	7.16	1538 ± 62.14	4.04	1578 ± 72.92	4.62
Calycosin-7-O-β-D-glucoside	15.15	14.53 ± 0.93	6.40	14.72 ± 1.34	9.11	14.05 ± 1.31	9.31
	75.75	77.08 ± 4.93	6.39	82.83 ± 7.14	8.62	78.27 ± 2.88	3.68
	151.5	149.2 ± 10.29	6.90	143.8 ± 7.73	5.38	141.4 ± 9.57	6.77
Calycosin-glucuronide	10.38	9.99 ± 0.67	6.69	9.87 ± 0.34	3.42	9.71 ± 0.46	4.69
	519	523.5 ± 19.84	3.79	557.9 ± 50.32	9.02	532.1 ± 25.91	4.87
	1038	1025 ± 78.08	7.62	1031 ± 45.24	4.39	1051 ± 50.02	4.76
Formononetin	5.23	5.47 ± 0.57	10.89	5.58 ± 0.37	6.65	5.35 ± 0.63	11.73
	261.5	245.4 ± 1162.01	13.92	261.5 ± 23.25	8.89	251.2 ± 27.17	10.82
	313.8	311.6 ± 28.6	9.18	322.7 ± 30.69	9.51	308.7 ± 25.75	8.34
Ononin	10.41	9.67 ± 0.73	7.51	9.9 ± 0.46	4.63	9.79 ± 0.74	7.61
	507	505.7 ± 34.19	6.76	469.0 ± 41.13	8.77	475.6 ± 22.73	4.78
	1014	993.2 ± 66.94	6.74	942.6 ± 51.09	5.42	920.9 ± 40.52	4.40
Glycyrrhizic acid	12.73	11.64 ± 1.28	11.01	12.26 ± 0.77	6.27	11.89 ± 1.3	10.97
	1273	1250 ± 68.62	5.49	1186 ± 83.68	7.06	1185 ± 83.68	7.06
	2545	2472 ± 139.15	5.63	2463 ± 104.69	4.25	2414 ± 74.35	3.08