Research Article
A Validated RP-HPLC Method for Simultaneous Determination of Cefixime and Clavulanic Acid Powder in Pediatric Oral Suspension
Table 1
Results of method optimization.
| | Column | Mobile phase | Elution mode | Flow rate | pH | Observation | Result |
| | C18 | Methanol-water consists of disodium hydrogen phosphate (20 : 80, v/v) | Isocratic | 1.0 mL/min | 6.5 | Poor resolution | Rejected | | C18 | Methanol-water consists of disodium hydrogen phosphate (20 : 80, v/v) | Isocratic | 1.0 mL/min | 6.0 | Poor resolution | Rejected | | C18 | Methanol-water consists of disodium hydrogen phosphate (20 : 80, v/v) | Isocratic | 1.0 mL/min | 5.5 | Good resolution | Accepted | | C18 | Methanol-water consists of disodium hydrogen phosphate (20 : 80, v/v) | Isocratic | 1.0 mL/min | 5.0 | Poor and unstable peak shape | Rejected | | C18 | Methanol-water consists of disodium hydrogen phosphate (20 : 80, v/v) | Isocratic | 1.0 mL/min | 4.5 | Poor and unstable peak shape | Rejected | | C18 | Methanol-water consists of disodium hydrogen phosphate (20 : 80, v/v) | Isocratic | 1.0 mL/min | 4.0 | Poor and unstable peak shape | Rejected |
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