International Journal of Analytical Chemistry

Research Article

Quantitative Identification of Antioxidant Basis for Dendrobium Nobile Flower by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Table 1

Correlation of antioxidant activities to metabolites in D. nobile.


NO.	Name	ABTS scavenging					DPPH scavenging					Ferric reducing					CSCF/TCCF (%)	CSCF/CSCA (%)
NO.	Name	100	200	500	1000	2000	100	200	500	1000	2000	100	200	500	1000	2000	CSCF/TCCF (%)	CSCF/CSCA (%)

1	DTFC-HBA	0.900^∗	0.937^∗	0.819	0.488	0.533	0.963^∗∗	0.939^∗	0.923^∗	0.666	0.496	0.607	0.766	0.797	0.864	0.894^∗	0.0023	45.82
2	1-aminocyclopentanecarboxylate	0.860	0.941^∗	0.798	0.728	0.656	0.889^∗	0.829	0.817	0.810	0.536	0.597	0.734	0.717	0.894^∗	0.846	0.0273	41.75
3	2-amino-1,3,4-tetradecanetriol	0.906^∗	0.829	0.892^∗	0.547	0.636	0.722	0.944^∗	0.932^∗	0.654	0.435	0.799	0.856	0.892^∗	0.838	0.902^∗	0.0004	24.19
4	2′-deoxycytidine 5′-diphosphate	0.865	0.906^∗	0.774	0.414	0.495	0.960^∗∗	0.912^∗	0.891^∗	0.602	0.446	0.569	0.738	0.773	0.830	0.867	0.0440	55.39
5	2-hydroxy-6-aminopurine	0.848	0.947^∗	0.778	0.680	0.528	0.936^∗	0.833	0.838	0.818	0.645	0.506	0.658	0.651	0.848	0.810	0.0732	31.79
6	2-methylglutaric acid	0.888^∗	0.962^∗∗	0.803	0.554	0.511	0.991^∗∗	0.908^∗	0.902^∗	0.733	0.578	0.546	0.715	0.733	0.863	0.866	0.0432	54.54
7	3-hydroxybutyrate	0.764	0.856	0.647	0.383	0.542	0.943^∗	0.783	0.736	0.514	0.267	0.487	0.688	0.699	0.808	0.800	0.0135	42.96
8	5-aminosalicylic acid	0.852	0.946^∗	0.758	0.541	0.500	0.991^∗∗	0.865	0.855	0.712	0.548	0.501	0.681	0.692	0.845	0.835	0.1050	69.70
9	5-oxoproline	0.759	0.864	0.644	0.422	0.529	0.945^∗	0.769	0.729	0.553	0.317	0.462	0.663	0.668	0.805	0.785	0.0054	48.62
10	6-aminohexanoic acid	0.821	0.916^∗	0.715	0.446	0.451	0.998^∗∗	0.849	0.834	0.635	0.494	0.462	0.656	0.676	0.810	0.813	0.0087	51.43
11	8-gingerol	0.837	0.934^∗	0.740	0.502	0.445	0.998^∗∗	0.858	0.853	0.694	0.569	0.462	0.648	0.664	0.816	0.813	0.0077	62.04
12	8-oxo-dGMP	0.769	0.820	0.658	0.330	0.613	0.888^∗	0.798	0.733	0.434	0.129	0.575	0.761	0.779	0.820	0.832	0.0003	36.85
13	9(S)-HpOTrE	0.860	0.867	0.778	0.378	0.539	0.902^∗	0.913^∗	0.882^∗	0.543	0.351	0.629	0.781	0.821	0.826	0.878	0.0009	56.95
14	Adipic acid	0.880^∗	0.956^∗	0.791	0.539	0.507	0.993^∗∗	0.901^∗	0.892^∗	0.719	0.562	0.536	0.710	0.727	0.858	0.861	0.0533	57.13
15	α-ketoglutaric acid	0.803	0.872	0.695	0.334	0.418	0.974^∗∗	0.855	0.832	0.538	0.416	0.470	0.663	0.699	0.775	0.807	0.0050	65.87
16	Angelol-K	0.837	0.939^∗	0.771	0.671	0.450	0.936^∗	0.828	0.848	0.838	0.731	0.458	0.606	0.604	0.809	0.779	0.0378	27.11
17	Astragalin	0.858	0.906^∗	0.766	0.412	0.466	0.967^∗∗	0.906^∗	0.890^∗	0.609	0.476	0.542	0.715	0.750	0.817	0.853	0.8209	96.06
18	Caffeic acid	0.859	0.903^∗	0.768	0.407	0.477	0.962^∗∗	0.907^∗	0.889^∗	0.600	0.457	0.553	0.724	0.760	0.820	0.858	1.3346	91.91
19	Caffeic acid O-glucoside	0.821	0.878	0.728	0.367	0.370	0.961^∗∗	0.877	0.873	0.593	0.527	0.468	0.643	0.684	0.759	0.801	0.3282	85.94
20	Calycosin-7-O-glucoside	0.837	0.861	0.743	0.345	0.517	0.920^∗	0.891^∗	0.856	0.515	0.327	0.588	0.756	0.795	0.813	0.860	0.0050	44.83
21	Cyanidin 3-O-glucoside	0.851	0.909^∗	0.758	0.415	0.443	0.976^∗∗	0.898^∗	0.886^∗	0.620	0.502	0.517	0.693	0.727	0.808	0.840	0.0366	87.84
22	Cyanidin O-acetylhexoside	0.854	0.902^∗	0.761	0.402	0.472	0.966^∗∗	0.902^∗	0.883^∗	0.596	0.455	0.544	0.718	0.754	0.817	0.853	0.0509	94.00
23	δ-Aminolevulinic acid-HCL	0.845	0.928^∗	0.745	0.463	0.471	0.997^∗∗	0.875	0.860	0.652	0.506	0.495	0.682	0.704	0.827	0.835	0.0434	53.72
24	D-glutamine	0.880^∗	0.957^∗	0.791	0.545	0.520	0.992^∗∗	0.898^∗	0.888^∗	0.718	0.550	0.542	0.716	0.731	0.864	0.864	4.7157	60.99
25	Diethanolamine	0.867	0.942^∗	0.817	0.779	0.652	0.866	0.830	0.827	0.858	0.597	0.605	0.726	0.706	0.891^∗	0.840	0.0179	37.61
26	Diosgenin	0.874	0.914^∗	0.784	0.431	0.513	0.961^∗∗	0.918^∗	0.897^∗	0.613	0.446	0.582	0.750	0.783	0.842	0.876	0.1529	46.12
27	DL-pecolinic acid	0.872	0.943^∗	0.800	0.678	0.693	0.904^∗	0.850	0.823	0.756	0.448	0.633	0.780	0.768	0.916^∗	0.878	3.2369	41.35
28	D-norvaline	0.840	0.930^∗	0.739	0.498	0.527	0.986^∗∗	0.856	0.833	0.657	0.461	0.513	0.701	0.713	0.848	0.841	2.2341	50.51
29	dUDP	0.863	0.907^∗	0.771	0.414	0.488	0.963^∗∗	0.910^∗	0.890^∗	0.603	0.452	0.561	0.732	0.766	0.827	0.863	0.0435	56.10
30	Hesperetin 5-O-glucoside	0.825	0.881^∗	0.730	0.366	0.392	0.964^∗∗	0.880^∗	0.871	0.586	0.501	0.482	0.659	0.698	0.770	0.811	0.0778	85.24
31	Hesperidin	0.844	0.892^∗	0.751	0.386	0.438	0.962^∗∗	0.896^∗	0.882^∗	0.592	0.476	0.522	0.695	0.734	0.797	0.837	0.0674	92.29
32	Isomucronulatol-7-O-glucoside	0.822	0.877	0.730	0.366	0.375	0.960^∗∗	0.879^∗	0.873	0.590	0.520	0.473	0.647	0.689	0.761	0.804	0.7168	87.56
33	Kaji-ichigoside F1	0.847	0.892^∗	0.753	0.384	0.462	0.960^∗∗	0.898^∗	0.878^∗	0.581	0.445	0.538	0.712	0.749	0.808	0.847	0.0218	91.17
34	Levodopa	0.844	0.894^∗	0.748	0.384	0.457	0.965^∗∗	0.895^∗	0.875	0.583	0.449	0.530	0.706	0.743	0.806	0.843	0.1576	80.70
35	L-isoleucine	0.827	0.919^∗	0.724	0.445	0.439	0.999^∗∗	0.858	0.846	0.642	0.515	0.463	0.654	0.677	0.807	0.814	5.2740	53.18
36	L-leucine	0.827	0.920^∗	0.724	0.449	0.442	0.999^∗∗	0.858	0.845	0.644	0.515	0.463	0.655	0.676	0.809	0.815	7.7092	53.34
37	L-serine	0.874	0.949^∗	0.818	0.753	0.662	0.885^∗	0.842	0.833	0.834	0.563	0.613	0.743	0.726	0.902^∗	0.855	0.1720	37.96
38	L-tyrosine	0.843	0.914^∗	0.750	0.434	0.405	0.986^∗∗	0.886^∗	0.883^∗	0.651	0.564	0.477	0.655	0.687	0.793	0.819	0.0469	74.40
39	L-valine	0.845	0.933^∗	0.744	0.493	0.518	0.990^∗∗	0.864	0.843	0.659	0.473	0.514	0.701	0.715	0.847	0.843	0.0953	52.93
40	Methylenesuccinic acid	0.874	0.963^∗∗	0.799	0.656	0.562	0.957^∗	0.867	0.863	0.796	0.600	0.547	0.704	0.703	0.876	0.848	0.0004	39.37
41	methylQuercetin O-hexoside	0.838	0.888^∗	0.744	0.378	0.427	0.962^∗∗	0.891^∗	0.878	0.586	0.477	0.512	0.687	0.725	0.790	0.830	0.4173	90.99
42	N2-methylguanosine	0.790	0.842	0.680	0.292	0.477	0.939^∗	0.844	0.803	0.468	0.287	0.516	0.706	0.742	0.783	0.820	0.0039	42.74
43	N7-methylguanosine	0.817	0.895^∗	0.715	0.386	0.392	0.989^∗∗	0.864	0.854	0.603	0.512	0.451	0.640	0.673	0.777	0.802	0.0088	52.97
44	N-acetyl-D-glucosamine	0.834	0.920^∗	0.734	0.513	0.588	0.961^∗∗	0.841	0.808	0.641	0.391	0.547	0.730	0.736	0.866	0.850	0.0017	49.51
45	N-acetylglycine	0.849	0.938^∗	0.752	0.497	0.474	0.998^∗∗	0.872	0.862	0.683	0.538	0.492	0.676	0.694	0.832	0.833	0.0018	64.55
46	Naringin	0.826	0.933^∗	0.734	0.541	0.427	0.988^∗∗	0.839	0.842	0.732	0.623	0.436	0.616	0.628	0.804	0.791	0.0015	56.10
47	Neocryptotanshinone	0.876	0.915^∗	0.792	0.441	0.479	0.959^∗∗	0.923^∗	0.911^∗	0.637	0.503	0.568	0.730	0.765	0.828	0.866	0.0040	52.11
48	p-Coumaric acid	0.857	0.901^∗	0.764	0.402	0.476	0.962^∗∗	0.906^∗	0.886^∗	0.596	0.453	0.551	0.723	0.759	0.818	0.856	0.6013	87.50
49	Phellodenol H O-hexoside	0.848	0.883^∗	0.752	0.375	0.527	0.942^∗	0.896^∗	0.862	0.544	0.351	0.584	0.757	0.792	0.830	0.868	0.0092	63.80
50	Phenylacetylglutamine	0.889^∗	0.943^∗	0.801	0.491	0.533	0.980^∗∗	0.922^∗	0.904^∗	0.667	0.490	0.582	0.752	0.777	0.866	0.885^∗	0.0517	73.27
51	Pipecolic acid	0.846	0.928^∗	0.779	0.706	0.668	0.882^∗	0.815	0.796	0.779	0.487	0.596	0.737	0.719	0.892^∗	0.842	0.6891	41.01
52	Plantagoside	0.904^∗	0.945^∗	0.824	0.504	0.538	0.968^∗∗	0.940^∗	0.925^∗	0.680	0.509	0.607	0.767	0.795	0.870	0.896^∗	0.0054	76.21
53	Polygalaxanthone VII	0.859	0.892^∗	0.768	0.393	0.499	0.946^∗	0.909^∗	0.885^∗	0.577	0.415	0.577	0.744	0.781	0.824	0.866	0.0233	82.41
54	Purine	0.793	0.912^∗	0.699	0.559	0.470	0.960^∗∗	0.790	0.783	0.715	0.556	0.429	0.611	0.610	0.804	0.771	0.0117	56.54
55	Quercetin	0.828	0.883^∗	0.736	0.375	0.386	0.963^∗∗	0.883^∗	0.877	0.597	0.521	0.481	0.655	0.695	0.769	0.810	0.0168	82.59
56	Quercetin 4′-O-glucoside	0.821	0.879^∗	0.727	0.369	0.369	0.964^∗∗	0.876	0.872	0.596	0.531	0.465	0.640	0.681	0.759	0.800	0.6651	85.22
57	Quercetin 5-O-hexoside	0.826	0.881^∗	0.732	0.370	0.385	0.963^∗∗	0.881^∗	0.874	0.591	0.514	0.479	0.654	0.695	0.768	0.809	0.0771	83.09
58	Quercetin O-malonylhexoside	0.841	0.881^∗	0.749	0.369	0.442	0.950^∗	0.896^∗	0.879^∗	0.572	0.452	0.533	0.703	0.743	0.793	0.838	0.0223	75.85
59	Quercetin-O-glucoside	0.834	0.890^∗	0.734	0.372	0.449	0.970^∗∗	0.884^∗	0.863	0.571	0.438	0.514	0.696	0.732	0.800	0.835	0.0355	79.23
60	Quercitrin	0.861	0.890^∗	0.767	0.425	0.637	0.920^∗	0.893^∗	0.844	0.548	0.266	0.653	0.819	0.843	0.878	0.901^∗	0.0070	52.83
61	Rhodomyrtone	0.876	0.912^∗	0.788	0.430	0.511	0.956^∗	0.921^∗	0.901^∗	0.613	0.450	0.587	0.752	0.786	0.840	0.877	0.0487	39.16
62	Rutin	0.852	0.898^∗	0.760	0.399	0.451	0.963^∗∗	0.902^∗	0.887^∗	0.601	0.478	0.533	0.705	0.742	0.806	0.845	2.4000	96.16
63	Scopolamine	0.809	0.855	0.708	0.314	0.422	0.946^∗	0.868	0.845	0.518	0.397	0.501	0.682	0.724	0.769	0.815	0.0702	80.18
64	Sibiricose A6	0.834	0.930^∗	0.738	0.492	0.420	0.998^∗∗	0.860	0.859	0.695	0.592	0.450	0.634	0.654	0.804	0.805	0.0016	55.05
65	Sibirioside A	0.827	0.881^∗	0.724	0.355	0.465	0.964^∗∗	0.877	0.850	0.547	0.395	0.521	0.705	0.741	0.802	0.836	0.0122	81.31
66	Threonyl carbamoyl adenosine	0.899^∗	0.976^∗∗	0.828	0.669	0.580	0.960^∗∗	0.894^∗	0.891^∗	0.809	0.609	0.580	0.732	0.733	0.894^∗	0.872	0.0039	39.87
67	Tiliroside	0.822	0.875	0.729	0.361	0.377	0.957^∗	0.879^∗	0.873	0.585	0.514	0.476	0.650	0.691	0.760	0.804	0.1808	87.70
68	trans-3-hydroxy-L-proline	0.803	0.899^∗	0.694	0.461	0.539	0.967^∗∗	0.815	0.782	0.603	0.378	0.496	0.690	0.699	0.833	0.819	0.0223	52.76
69	Tricetin O-hexoside	0.832	0.885^∗	0.740	0.377	0.397	0.961^∗∗	0.887^∗	0.879^∗	0.596	0.512	0.491	0.664	0.704	0.775	0.816	0.0664	82.69
70	Trifolin	0.851	0.910^∗	0.757	0.417	0.443	0.979^∗∗	0.897^∗	0.885^∗	0.622	0.504	0.514	0.691	0.725	0.809	0.840	0.0742	86.54
71	Vanillic acid	0.865	0.904^∗	0.780	0.420	0.462	0.956^∗	0.915^∗	0.903^∗	0.620	0.495	0.554	0.718	0.756	0.815	0.855	0.0085	86.75
72	Xanthosine	0.932^∗	0.914^∗	0.873	0.530	0.699	0.869	0.962^∗∗	0.926^∗	0.639	0.350	0.772	0.893^∗	0.919^∗	0.919^∗	0.957^∗	0.0054	42.84

CSCF/TCCF the contents of one specific component in flower to the total contents of 712 components in flower. CSCF/CSCA the contents of one specific component in flower to the contents of this component in all of root, stem, leaf, flower, and fruit. DTFC-HBA (2S, 3R)-2-{[{9-[(2R, 3R, 4S, 5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydro-2-furanyl]-9H-purin-6-yl}(methyl)carbamoyl]amino}-3-hydroxybutanoic acid, 9(S)-HpOTrE (9S, 10E, 12Z, 15Z)-9-Hydroperoxy-10, 12, 15-octadecatrienoate.