Analytical Validation of Smartphone Spectroscopic Technic Used in an Educational Kinetic Study

Table 4

Recapitulation of validation steps.

Characteristic	Test	Description of the test
Random character	Shapiro–Wilk test conform	For the risk α = 5%, R, and 12 repetitions, the number of sequences R = 8 is between higher and lower values.
Specificity	Specific method	Response of solutions prepared in water and in reaction mixture are equal.
Precision	Cochran test conform	There is no aberrant responses in the 12 measurements; the fraction of higher variance to the sum of variances is less than Cochran critical vale at the risk α = 5% and α = 5%.
Linearity	Fisher test conform	Fisher test showed that the fraction of calculated residuals and experimental ones is inferior to the tabulated Fisher value for 5 levels repeated 3 times.
Calibration function	A = 1000 C + 0.002	Least square regression is involved to determinate the slop and the intercept of the calibration curve.
Correlation coefficient	0.9999	This value represents the fraction of the variation in one variable that may be explained by the other variable.
Limit of detection	mol·L^−1	Statistical test of comparison of the response at the value 0 becomes significant. The limit of detection is determined with a risk of 0.05%.
Limit of quantification	mol·L^−1	Statistical test of comparison of the response at the value 0 becomes significant. The limit of quantification is determined with a risk of 0.05%.
Uncertainty	mol·L^−1	Because of the difference between the real value and the measured one, a degree of uncertainty will pertain to measurement. Uncertainty is the absolute range in which measured value can be accepted.