Rapid Determination of Three Organic Acids in Polygonum Vivipari Rhizoma via One Marker by HPLC-UV at Equal Absorption Wavelength and Effervescence-Assisted Matrix Solid-Phase Dispersion

Table 4

Robustness tests for the sample solution.

Parameters		RRTs		Content (mg/g)
		Neochlorogenic acid	Cryptochlorogenic acid	Neochlorogenic acid	Cryptochlorogenic acid
Current method		0.438	1.397	4.63	0.78
Flow rate (mL/min)	0.9	0.439	1.394	4.55	0.77
	1.1	0.440	1.391	4.55	0.77
Column temperature (°C)	32	0.428	1.429	4.59	0.77
	38	0.448	1.365	4.51	0.79
Column	B15046	0.434	1.375	4.56	0.76
	B19476	0.442	1.387	4.59	0.77
Instrument	Agilent 1260 I	0.442	1.385	4.60	0.77

RRT was calculated as the retention time of the tested compound/the retention time of chlorogenic acid. Content was calculated based on the peak area of the tested compound/peak area of chlorogenic acid × concentration of chlorogenic acids.