Method Validation and Measurement Uncertainty (MU) Evaluation on Enrofloxacin and Ciprofloxacin in the Aquatic Products

Table 1

Different conditions of sample pretreatment experiments.

Item	Condition 1	Condition 2	Condition 3	Condition 4	Condition 5
Dosages of Na2SO4 (g)^①	0	5	10	15	20
Acidic acetonitrile with the composing proportion of 50% hydrochloric and acetonitrile (v/v)^②	0	4‰	8‰	12‰	16‰
Ultrasonic time (min)^③	0	5	10	20	30
Extraction (stand) time (min)^④	0	10	20	25	30
Dosages of n-hexane (mL)^⑤	0	2	—	—	—

Note. In sample pretreatment experiments, ^①means the corresponding weights of Na₂SO₄ including 0 g, 5 g, 10 g, 15 g, and 20 g were added to be dehydrated for samples; ^②means 20 mL of acidic acetonitrile used for extraction reagent which was composed with the different proportion of 0, 4%, 8%, 12%, and 16% with both acetonitrile and 50% hydrochloric (v/v); ^④means the step of ultrasonic bath time for 0 min, 5 min, 10 min, 20 min, and 30 min; ^⑤means the step of extracted (stand) time for 0 min, 10 min, 20 min, 25 min, and 30 min; ^⑥ means the step on getting rid of the fat by using 0 mL or 2 mL of n-hexane.