Method Validation and Measurement Uncertainty (MU) Evaluation on Enrofloxacin and Ciprofloxacin in the Aquatic Products

Table 3

The result of sample optimal extraction and treatment.

Item	Optimal extraction conditions
Dosages of Na2SO4 (g)^①	15
Acidic acetonitrile with the composing proportion of 50% hydrochloric and acetonitrile (v/v)^②	8‰
Ultrasonic time (min)^③	10
Extraction (stand) time (min)^④	20
Dosages of n-hexane (mL)^⑤	2

Note. In sample pretreatment experiments, ^①means the corresponding weights of Na₂SO₄ of 15 g were added to be dehydrated for samples; ^②means 20 mL of acidic acetonitrile used for extraction reagent which was composed with the proportion of 8% with both acetonitrile and 50% hydrochloric (v/v); ^③means the step of ultrasonic bath time for 10 min; ^④means the step of extracted (stand) time for 20 min; ^⑤means the step on getting rid of the fat by using 2 mL of n-hexane.