Bioassay and RP-HPLC Method Development for the Analysis of Extracts and Herbal Products Containing Anthocleista nobilis

Table 2

Percentage yield and antioxidant activity of the extracts obtained from the stem bark of Anthocleista nobilis.

Test	% yield (% w/w) ± SD	EC50 (μg/mL)	TAC (μg/g AAE) ± SD
Water	9.29 ± 0.03	48.37	439.34 ± 44.11
70% ethanol	18.14 ± 0.08	97.69	557.04 ± 24.39
96% ethanol	18.67 ± 0.10	66.66	629.60 ± 51.87
Ethyl acetate	5.60 ± 0.14	36.39	632.64 ± 23.13
Control (ascorbic acid)		11.82

All results are means of triplicate determinations.